Stable Isotopes in Custom Peptide Synthesis
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Stable Isotopes in Custom Peptide Synthesis
Stable Isotopes in Custom Peptide Synthesis
Isotopes refer to differences in the number of neutrons in the nucleus of atoms; isotopes can be radioactive, when they decay, releasing energy in the process and stable isotopes, as the name indicates, do not decay and actually can be isolated as such. Most biologically active proteins have two or more stable isotopes, with the lightest ones representing the most abundant ones. For example C13 and N15 are heavy isotopes with an abundance of 1% and C12 and N14 have an abundance of 99%.. In the case of carbon, there is also a radioactive form C14.Stable isotopes can be introduced in to amino acids, by feeding growing bacteria with the corresponding precursor, containing the desired element. In turn these amino acids can be used to prepare the corresponding Fmoc protected amino acids, that then can be incorporated during Peptide Synthesis into specific positions of the given peptide/protein.
MALDI-TOF of small peptides may not easily detect the difference in mass, but in larger proteins one can more readily detect the differential mass distribution due to the presence of the heavy isotope. The biological researcher can introduce at will stable isotopes into the sequence of their interest, and this is very helpful in studying protein-protein interactions,using methods, such us proton NMR as an example. One challenge in using heavy/stable isotopes is their high cost, due to the cost involved during the enrichment process. Most masses given for Peptides correspond to the calculated monoisotopic masses. For refined and/or special applications, the biological researcher will need to take into account the heavy isotope masses, and as we mention before, the heavier the protein the more noticeable the increased differential in the molecular weight of the protein in question. Synthetic Peptide chemistry has proven very valuable as it allows the production of milligram to gram quantities of stable isotope containing peptides, where selected amino acids can at will contain the corresponding stable isotopes of carbon, hydrogen or nitrogen.
Please visit our Blog on Blogspot Bio-Synthesis
Isotopes refer to differences in the number of neutrons in the nucleus of atoms; isotopes can be radioactive, when they decay, releasing energy in the process and stable isotopes, as the name indicates, do not decay and actually can be isolated as such. Most biologically active proteins have two or more stable isotopes, with the lightest ones representing the most abundant ones. For example C13 and N15 are heavy isotopes with an abundance of 1% and C12 and N14 have an abundance of 99%.. In the case of carbon, there is also a radioactive form C14.Stable isotopes can be introduced in to amino acids, by feeding growing bacteria with the corresponding precursor, containing the desired element. In turn these amino acids can be used to prepare the corresponding Fmoc protected amino acids, that then can be incorporated during Peptide Synthesis into specific positions of the given peptide/protein.
MALDI-TOF of small peptides may not easily detect the difference in mass, but in larger proteins one can more readily detect the differential mass distribution due to the presence of the heavy isotope. The biological researcher can introduce at will stable isotopes into the sequence of their interest, and this is very helpful in studying protein-protein interactions,using methods, such us proton NMR as an example. One challenge in using heavy/stable isotopes is their high cost, due to the cost involved during the enrichment process. Most masses given for Peptides correspond to the calculated monoisotopic masses. For refined and/or special applications, the biological researcher will need to take into account the heavy isotope masses, and as we mention before, the heavier the protein the more noticeable the increased differential in the molecular weight of the protein in question. Synthetic Peptide chemistry has proven very valuable as it allows the production of milligram to gram quantities of stable isotope containing peptides, where selected amino acids can at will contain the corresponding stable isotopes of carbon, hydrogen or nitrogen.
Please visit our Blog on Blogspot Bio-Synthesis
BIO-SYNTHESIS
BIO-SYNTHESIS, INC., is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories.
Please visit our Blog on Blogspot Bio-Synthesis
Please visit our Blog on Blogspot Bio-Synthesis
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"The use of state-of-the art technology has allowed Bio-Synthesis, Inc. to rapidly gain recognition for its manufacturing quality & dependability of delivery."
Founded in 1984, Bio-Synthesis, Inc. was first known as OCS Laboratories. It was the first producer of commercially available synthetic DNA and, in 1985, became a producer of synthetic peptides. In 1989, OCS incorporated as Bio-Synthesis, Inc. and moved its laboratories to Lewisville, Texas. Today, BSI occupies 10,000 square feet of modern laboratory space (completed in 1995) located 20 miles from downtown Dallas and 10 miles from D/FW airport. Among the surrounding academic institutions are: University of Texas Southwestern Medical School (12 miles), University of North Texas (10 miles), and University of North Texas Health Science Center (25 miles). These universities provide excellent library resources. Since its inception, BSI has steadily expanded its product lines and services to meet the growing needs of the molecular biology community. It has maintained its position as an aggressive, innovative company in a highly competitive marketplace without sacrificing quality. In the beginning, our primary emphasis was on synthesizing high quality DNA primers and linkers, which were the initial uses of oligos. Today, newer technologies, such as gene construction, PCR, mutagenesis, combinatorial libraries, dye/adduct labeling, DNA microarrays, peptide-nucleic acid chimeras, etc. have challenged the molecular biology field. In response, BSI has branched into several related areas including DNA paternity testing, DNA HLA typing, PNA synthesis, genomic sequencing, fluorescence-based genotyping, custom organic synthesis and other molecular biology based applications.
The Research and Development Division comprises a number of Ph.D. and M.D. professionals with backgrounds in molecular biology, immunology, nucleic acids, peptide synthesis, general organic chemistry, and automated amino acid sequencing. The Molecular Biology Division is set up to handle most modern experimental procedures; the Immunology Division specializes in immunodiagnostic-based reagents.
Not only has BSI continued to provide quality DNA products and services for the research community, but it has also become a world leader in providing custom peptide products and services. Using state-of-the-art solid phase peptide chemistries, BSI provides high quality custom peptides, performs carrier conjugations, antipeptide antibody production, antigenic peptide design, synthesis of long and/or modified peptides, MALDI TOF analysis, contract research, consultation services and more.
Biomedical researchers worldwide in universities, biotech companies, private clinics, and government agencies use products from Bio-Synthesis, Inc. in studies ranging from PCR diagnostics to cancer research and the Human Genome Project. Contact us to discover how BSI can customize products/services to suit your research requirements.
For more detail please visit our About Us
Please Contact Us :
Address: 612 E. Main street
City: Lewisville
State: Texas
Country: USA
Postal Code: 75057
Phone Number: 972-420-8505
Founded in 1984, Bio-Synthesis, Inc. was first known as OCS Laboratories. It was the first producer of commercially available synthetic DNA and, in 1985, became a producer of synthetic peptides. In 1989, OCS incorporated as Bio-Synthesis, Inc. and moved its laboratories to Lewisville, Texas. Today, BSI occupies 10,000 square feet of modern laboratory space (completed in 1995) located 20 miles from downtown Dallas and 10 miles from D/FW airport. Among the surrounding academic institutions are: University of Texas Southwestern Medical School (12 miles), University of North Texas (10 miles), and University of North Texas Health Science Center (25 miles). These universities provide excellent library resources. Since its inception, BSI has steadily expanded its product lines and services to meet the growing needs of the molecular biology community. It has maintained its position as an aggressive, innovative company in a highly competitive marketplace without sacrificing quality. In the beginning, our primary emphasis was on synthesizing high quality DNA primers and linkers, which were the initial uses of oligos. Today, newer technologies, such as gene construction, PCR, mutagenesis, combinatorial libraries, dye/adduct labeling, DNA microarrays, peptide-nucleic acid chimeras, etc. have challenged the molecular biology field. In response, BSI has branched into several related areas including DNA paternity testing, DNA HLA typing, PNA synthesis, genomic sequencing, fluorescence-based genotyping, custom organic synthesis and other molecular biology based applications.
The Research and Development Division comprises a number of Ph.D. and M.D. professionals with backgrounds in molecular biology, immunology, nucleic acids, peptide synthesis, general organic chemistry, and automated amino acid sequencing. The Molecular Biology Division is set up to handle most modern experimental procedures; the Immunology Division specializes in immunodiagnostic-based reagents.
Not only has BSI continued to provide quality DNA products and services for the research community, but it has also become a world leader in providing custom peptide products and services. Using state-of-the-art solid phase peptide chemistries, BSI provides high quality custom peptides, performs carrier conjugations, antipeptide antibody production, antigenic peptide design, synthesis of long and/or modified peptides, MALDI TOF analysis, contract research, consultation services and more.
Biomedical researchers worldwide in universities, biotech companies, private clinics, and government agencies use products from Bio-Synthesis, Inc. in studies ranging from PCR diagnostics to cancer research and the Human Genome Project. Contact us to discover how BSI can customize products/services to suit your research requirements.
For more detail please visit our About Us
Please Contact Us :
Address: 612 E. Main street
City: Lewisville
State: Texas
Country: USA
Postal Code: 75057
Phone Number: 972-420-8505
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BIO-SYNTHESIS
BIO-SYNTHESIS, INC., is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories.
Please visit our Blog on Blogspot Bio-Synthesis
Please visit our Blog on Blogspot Bio-Synthesis
Custom Peptides
As a leading supplier of genomic and proteomic research products, BSI is able to offer its customers integrated solutions for custom DNA synthesis, custom RNA synthesis, custom polyclonal antibody production, custom peptide synthesis, molecular diagnostic detection kits, DNA Real-Time PCR products, DNA microarray services and a wide range of custom DNA-based diagnostic kits and consumables.
The proteomic division is experienced in the design of peptides, both for antibody production and bioactive peptide synthesis, e.g cosmetic peptides. While the custom peptide synthesis division has the ability to produce thousands of modified or non-modified peptides per day for research applications, BSI also provides diagnostic/therapeutic GMP custom peptide production. Synthesis of custom peptides results in quantities ranging from milligrams to multi-kilograms. The staff in the custom antibody division provide expert support in the selection of peptide antigens for target antibody production and offer convenient custom-bundled packages with a wide range of host animals. In addition, peptide, antibody and protein arrays are also amongst its expertise.
BSI is a U.S. based company whose primary emphasis is on the synthesis of high quality custom products for a variety of applications and a full-service contract manufacturing organization (CMO) delivering research, development and production services for cGMP clinical batch manufacturing.
BIO-SYNTHESIS
BIO-SYNTHESIS, INC., is a leading life science products company with over 20 years of experience in the design and synthesis of Custom Peptide, small molecules and reagents for small scale research and bulk pharmaceutical trials. Using state of the art technology in our well-equipped laboratories.
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